• Users Online: 75
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login
Export selected to
Reference Manager
Medlars Format
RefWorks Format
BibTex Format
   Table of Contents - Current issue
September-December 2016
Volume 1 | Issue 3
Page Nos. 31-99

Online since Thursday, February 2, 2017

Accessed 1,093 times.

PDF access policy
Journal allows immediate open access to content in HTML + PDF
View as eBookView issue as eBook
Author Institution MappingAuthor Institution Mapping
Access StatisticsIssue statistics
Hide all abstracts  Show selected abstracts  Export selected to  Add to my list

Real-time quantitative polymerase chain reaction detection of minimal residual disease in acute lymphoblastic leukemia: a single-center experience p. 31
Shabaan Redwaan, Hesham Abdelraheem, Taghreed K Eldin, Hosny Badrawy, Eman M Nagiub Abdelsalam
Background Monitoring of minimal residual disease (MRD) has become a frontline clinical practice in the treatment of virtually all childhood acute lymphoblastic leukemia (ALL) cases and in many cases of adult patients with ALL. The MRD diagnostics has proven to be the strongest prognostic factor allowing for risk group assignment into different treatment arms. The MRD techniques need to be sensitive (≤10–4), which means, the ability to detect one malignant cell among 10 000 normal cells; broadly applicable; accurate; reliable; fast; and affordable. Aim The objective of this study is to evaluate the analysis of immunoglobulin heavy chain (IGH) or T-cell receptor (TCR) gene rearrangements as targets for MRD assessment in ALL, allowing early detection of relapsed cases, compare with the results of morphological evaluation of the same cases and to risk stratify patients with ALL according to the MRD assessment as a prognostic marker independent and superior to other conventional risk factors. Patients and methods Overall, 30 patients (15 males and 15 females) with age ranged from 1 to 25 years old were included in this study. Patients were subjected to full medical history, clinical examination, laboratory examinations such as complete blood count, bone marrow aspirate smear examination, cytochemistry, and immunophenotyping. The molecular studies done by real-time PCR were performed using consensus primers and allele-specific primers for (IGH) or (TCR) gene rearrangements as targets to detect MRD. The ALL cases were assessed by real-time quantitative PCR at the time of diagnosis and at the end of induction chemotherapy, and comparative cycle threshold (Ct) relative quantification method was used for quantitative gene expression. Results The results showed discrepancy between the morphologic examination for ALL assessment depending on the 5% blast index as an indicator of remission or nonremission; morphological nonremissions (>5% blast) were detected in eight (26.7%) cases whereas 22 (73.3%) cases showed morphological remissions (<5% blast). Real-time quantitative PCR for IGH/TCR gene rearrangements was done for 30 patients. Of the 22 cases that showed morphological remission, there were 18 (81.8%) cases which were MRD positive (i.e. not in molecular remission) and four (18.2%) cases which were MRD negative (i.e., in molecular remission). Patients who showed nonremission morphologically (right cases) were confirmed to be MRD positive. None of the patient's clinical variables such as age, sex, total leukocyte count, and ALL immunophenotype have been identified as predictors of MRD risk. Conclusion Approximately 81% of ALL cases previously diagnosed as being in the remission state depending on the morphologic assessment only tend to harbor MRD as evident by positive MRD assessment using real-time PCR. The molecular assessment of MRD allows early detection of relapse with chance of intervention and tailoring of treatment according to the patients' need. It was found that there was no relation between MRD results and risk stratification in ALL according to age and white blood cell at diagnosis; this confirms that detection of MRD of leukemic cells can be considered a superior prognostic marker of relapse, independent from conventional prognostic factors. The risk classification in ALL based on MRD results could be used to guide the final treatment strategy and predict early relapse; this should allow prediction of response and relapse while patients are still in the state of clinical remission and morphological remission too.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

Construction of an Arabic computerized cognitive skill battery for the diagnosis of children with specific learning disabilities p. 38
Wafaa M. A. Farghaly, Mohammed A Ahmed, Taha A. H. Elmestikawy, Reda B Abdel-Rasool, Mohammed S. H. Farghaly, Montser S Omar, Amr S Ramadan, Mohammed Salamah, Mohammed A Abdel-Hameed, Adel T Mohammed
Objective Most of the previously designed Arabic tests for the assessment of specific learning disabilities (SLDs) are noncomputerized and do not pay attention to many of the cognitive skills that are believed to have an important role in the early stages of literacy development and its relations with learning disabilities. Computer usage supports and enhances children's creativity, self-esteem, and cooperative learning. Therefore, we aimed to design an Arabic computerized cognitive skill battery for the detailed assessment of children with SLD, through the assessment of their auditory as well as visual processing skills, to identify their points of weakness that might contribute to their learning disability. Participants and methods This study was carried out in Assiut city, Egypt. All fourth grade students from six chosen public primary schools (n = 858 students) were interviewed except those who were absent during their school visits (n = 142 students), or those who did not complete the test battery (n = 56 students). The rest of the sample (660 students) completed the test battery. The study included four stages. The first stage included identification of students with SLD with previous standardized tests [the Arabic reading test, the writing test (it is the sum of the script part of visuomotor test, which was specifically designed for this study, plus spelling part of Arabic reading test), the Math test, and the Wechsler Intelligence Scale for Children-Revised to assess their IQ level]. The second stage included battery construction by seven expert staff members (this took 1 year from 1 October 2012 to 30 September 2013). The third stage included application of the newly constructed battery in a pilot study to 10 normal students (9–10-year-old) to ascertain clarity of the tests of the battery. The fourth stage included test standardization. Results The reliability of the battery was proven using Cronbach's α correlation coefficient (α≥0.7), interitem correlations (all values are positive and highly significant), and corrected item-total correlation coefficient (all values >0.3). Validity was proven with judgment validity, construct validity (factorial analysis) (all loadings ≥0.5), contrasted group validity, and predictive validity (sensitivity and specificity). Conclusion The constructed battery was thus proven to be highly reliable and valid for the assessment of SLDs among Arabic reading children, and thus remediation programs can be directed properly and early.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

Anti-C1q antibodies as a marker for disease activity in systemic lupus erythematosus and lupus nephritis p. 47
Eman A.H. Omran, Nadia M Ismail, Eman Mosad, Yasmine S Hussein
Background Systemic lupus erythematosus (SLE) is a chronic multisystem inflammatory autoimmune disease that is characterized by a number of immunological abnormalities. Disease onset is triggered by ill-defined environmental factors in genetically susceptible individuals. C1q plays a key role in apoptotic cell and immune complex removal, and hence it is a very important functional molecule in SLE pathogenesis. Investigation of the relationship between peripheral lymphocyte apoptosis and serum levels of anti-C1q autoantibodies in SLE patients suggests that increased serum levels of anti-C1q autoantibodies are responsible for apoptosis and may play a pathogenic role in SLE patients, especially in active disease. Objectives The aim of this study was to measure the serum level of anti-C1q in SLE patients, and to evaluate the correlation between anti-C1q and SLE disease activity, especially renal activity. Patients and methods Fifty SLE patients diagnosed according to the Systemic Lupus International Collaborating Clinics classification criteria 2012 and 33 healthy volunteers who were age and sex matched were included in the study. SLE activity was assessed using the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI), and renal activity was assessed using the renal SLEDAI. Anti-C1q was estimated using enzyme-linked immunosorbent assay kit. Results Serum anti-C1q was significantly higher in SLE patients (64.86 ± 27.88 U/ml) compared with healthy controls (30.15 ± 13.93 U/ml) (P < 0.000). There was a significantly positive correlation between anti-C1q and the SLEDAI (P = 0.035, r = 0.299) and the renal SLEDAI (P = 0.025, r = 0.316). Anti-C1q has a sensitivity and specificity of 92.7 and 66.7%, respectively, a positive predictive value of 92.7%, negative predictive value of 66.7%, and 88.0% accuracy for detecting SLE disease activity, whereas for lupus nephritis diagnosis anti-C1q has a sensitivity and specificity of 94.12 and 50.0%, respectively, a positive predictive value of 80.0%, negative predictive value of 80.0%, and 80.0% accuracy. Conclusion Our results support the finding that anti-C1q level might be used as a marker for SLE activity and not lupus nephritis in adult SLE patients.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

Differential expression of MOC-31, Hep Par 1, and N-cadherin in primary carcinoma and metastatic adenocarcinoma in the liver p. 54
Mohammed A Ahmed, Fatma A Badary, Etemad H Yassin, Said A Mohammed, Madiha M El-Attar
Background Immunohistochemistry plays a crucial role in the diagnosis of hepatocellular carcinoma (HCC) and in its distinction from other primary and metastatic neoplasms. In this study, we examined the expression of MOC-31 (Anti-epithelial cell adhesion molecule monoclonal antibody, clone number-31), hepatocyte paraffin 1 (Hep Par 1), and N-cadherin in primary carcinoma and metastatic adenocarcinoma (AC) in the liver. Aim The aim of this study was to evaluate the usefulness of MOC-31, Hep Par 1, and N-cadherin in the differential diagnosis of primary carcinoma and metastatic AC in the liver. Materials and methods The present study included 56 specimens from cases of primary and metastatic liver tumors, including 20 primary HCCs in the liver, five intrahepatic cholangiocarcinomas, and 31 metastatic ACs in the liver. They were studied to evaluate MOC-31, Hep Par 1, and N-cadherin expression using immunohistochemistry. Results The sensitivity of MOC-31 for AC in the studied group was 97.2%, whereas its specificity was 90%. The sensitivity of Hep Par 1 for HCC was 75%, whereas its specificity was 100%. The sensitivity of N-cadherin for primary liver carcinoma was 72%, whereas its specificity was 83.9%. Using the combination of the three antibodies, a final diagnosis could be established in 52 of 56 (92.9%) cases of studied group. In conclusion, a panel of these three antibodies can be helpful in the distinction between primary carcinoma and metastatic AC in the liver.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

Prevalence of respiratory disorders among primary aluminum workers, and their relation to the duration of exposure and smoking habits p. 61
Hala H Aboufaddan, Hussein H Zayet, Lamiaa H Shaaban, Ashraf A Ewis, Shimaa A Elghazally
Introduction and study methods A cross-sectional study was conducted to describe the pattern of respiratory disorders and some associated risk factors among 260 aluminum-exposed workers and 80 nonexposed workers. Data were collected by a respiratory symptoms questionnaire. Spirometry and chest radiography were performed on all participants. Results The aluminum-exposed workers suffered significantly more from chronic cough, phlegm, wheezes and breathlessness (34.2, 42.3, 42.3 and 36.9%, respectively) compared with nonexposed workers (P < 0.05). Mean values of spirometric measurements regarding FVC%, FEV1%, FEV1/FVC and PEF25–75% as percentages of predicted were significantly lower among exposed workers (84.15 ± 15.02, 88.77 ± 17.65, 106.15 ± 14.72 and 81.34 ± 29.1, respectively) compared with nonexposed workers (P < 0.05).The interpretation of chest radiographies revealed that abnormal chest rdaiographies were significantly more predominant in exposed workers than in the nonexposed group (P < 0.05). There was a significantly higher occurrence of asthma among exposed workers (8.1%) compared with nonexposed workers (1.2%), with P value 0.03. The prevalence of respiratory symptoms, abnormal ventilatory function measurements and abnormal radiographies were higher among the exposed workers who were smokers and in those with duration of exposure of more than 25 years. Conclusion The occurrence of respiratory symptoms, airway diseases, ventilatory function abnormalities and abnormal rdaiographies were higher among the exposed workers, especially those who were smokers and had a duration of exposure of more than 25 years. For prevention of these hazards, general and personal measurements must be performed, for example, engineering, personal protective equipments and more medical care for workers (pre-employment and periodic medical examinations).
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

Prevalence of microscopic colitis in Upper Egypt among patients with chronic diarrhea and patients with irritable bowel syndrome p. 67
Omar M Saleh, Ashraf M Osman, Madiha M Al-Attar, Abeer M Rifai
Aim The aim of this study was to evaluate the prevalence of microscopic colitis (MC) in patients with chronic watery diarrhea and irritable bowel syndrome–diarrhea (IBS-D) predominant. Patients and methods We studied 61 patients with chronic nonbloody diarrhea and normal endoscopic findings prospectively for 2 years' duration; 37 patients had chronic watery diarrhea for investigation and 24 had IBS-D predominant. Results Our results showed that 18 (29.5%) patients had MC, 10 (16.4%) patients had collagenous colitis, and eight (13.1%) had lymphocytic colitis. Four (16.7%) patients out of the 24 patients with IBS-D predominant had MC. Abdominal pain, nocturnal diarrhea, and weight loss are the most common clinical presentation in MC. Conclusion MC is not uncommon in Upper Egypt patients with chronic watery diarrhea and normal colonoscopic findings. Biopsy of normal colonic mucosa in patients with chronic watery diarrhea is emphasized to reach to definite diagnosis of MC.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

Novel molecular diagnostic marker in the evaluation of cartilage destruction in patients with rheumatoid arthritis p. 72
Ghada Hassan, Elbadry Aboelnour, Osama Ibrahiem, Ebtsam F Mohammed
Aim The aim of this study was to evaluate the serum levels of cartilage oligomeric matrix protein (COMP) in rheumatoid patients in correlation with disease severity and cartilage destruction and to evaluate the therapeutic effectiveness of slow-remitting agents such as leflunomid on this marker of cartilage destruction. Patients and methods Fifty patients with rheumatoid arthritis (RA) diagnosed on the basis of the 2010 ACR/EULAR Rheumatoid Arthritis Classification Criteria and 20 age-matched and sex-matched controls were enrolled in the study. C-reactive protein, erythrocyte sedimentation rate (ESR), rheumatoid factor, anti- cyclic citrullinated peptide, and COMP were determined. Patients were classified into two groups according to Disease Activity Score-28: group 1 (29 patients) included patients with severe activity with a score of greater than 5.1; and group 2 (21 patients) included patients with moderate activity with a score of greater than 3.2 and less than and equal to 5.1. The studied patients were classified into two groups on the basis of the time of receiving leflunomid therapy (20 mg daily after initial therapy 100 mg daily for 3 days) for 3 months: group 3 received before treatment and group 4 received after treatment. Results Serum COMP was significantly higher in patients with RA when compared with controls (P = 0.000). The COMP levels were found to be positively correlated with Joint space narrowing score (JSN) (r = 0.832, P = 0.000) and erosion score (r = 0.863, P = 0.000) of radiography and negatively correlated with rheumatoid factor (r=−0.313, P = 0.027); however, COMP levels did not correlate with age (r = 0.231, P = 0.106), duration of disease (r = −0.060, P = 0.678), Disease Activity Score-28 (r = −0.098, P = 0.498), C-reactive protein (r = −0.242, P = 0.090), ESR first hour (r = −0.096, P = 0.509), ESR second hour (r = −0.101, P = 0.484), or anti-cyclic citrullinated peptide (r = 0.041, P = 0.775). Conclusion COMP could be a useful biomarker for the detection of early cartilage and bone destruction and in the follow-up of disease severity and treatment in RA.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

The nephroprotective effects of ginkgo biloba extract (EGb761) against l-NG-nitroarginine methyl ester-induced hypertension in rats: role of oxidative stress and inflammatory markers p. 79
Ahmed M Abd-Eldayem, Hanan S. M. Farghaly, Ahmed O Abdel-Zaher
Background Ginkgo biloba extract 761 (EGb761) was studied for its nephroprotective effects in experimentally induced hypertension. Hypertension is increasingly a cause of end-stage renal diseases. Increased cytokine release and oxidative stress are mechanisms that appear to be involved in the pathogenesis of hypertensive renal damage. EGb has antioxidant and anti-inflammatory effects and can attenuate hypertensive renal damage. Methods and results Male adult Wistar rats were used in this study. Hypertension was induced in these rats by administering l-NG-nitroarginine methyl ester (l-NAME) (10 mg/kg/day, intraperitoneal) for 12 weeks. Another group of rats received l-NAME and EGb761 (100 mg/kg/day, orally) starting from the ninth week to the end of treatment. It was found that the blood pressure was reduced at the end of 12th week in rats treated with EGb761 compared with l-NAME-treated (hypertensive) rats. EGb761-treated rats showed lower renal tissue malondialdehyde level and renal tissue tumor necrosis factor-α level when compared with l-NAME-treated rats (hypertensive). Conclusion EGb761 has antihypertensive effect; it can protect the kidney from hypertension through the reduction of renal inflammation and oxidative stress.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

Remediation of central auditory processing disorders in children with learning disability: a comparative study p. 86
Samah M Ahmed, Somaia M Tawfik, Mohamed S Bakr, Emad K Abdelhaleem, Enass S Mohamed
Objective The primary objective of this study was to compare the outcome of central auditory processing rehabilitation when using two different strategies. In the first strategy, the computer-based remediation program was used (temporal processing and phonemic awareness training). In the second strategy, the informal remediation program was used (temporal processing and phonemic awareness training). Patients and methods Fifty children with learning disability due to central auditory processing disorder were selected from primary schools in Assiut city. They were subjected to psychophysical test battery. It comprised dichotic digits test, pitch pattern sequence test, and electrophysiological test (cortical P1). They were divided into two equal groups, groups A and B. Each group was subdivided on the basis of age into three subgroups. Group A received the formal Auditory Training (AT) and group B received the informal AT for a minimum duration of 2 months. Re-evaluation of those children was carried out using the same test battery used in the diagnosis. Results The children were evaluated 1 month after training. There was a statistically significant difference in all psychophysical tests and electrophysiological P1. Subjective improvement was noticed also at the level of the questionnaire and school reports. Conclusion Both formal and informal remediation material used in this study proved to be effective and promising auditory training strategy for ameliorating central auditory disorder by remodeling the plasticity of the CANS.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta

Magnitude and pattern of maternal near-miss cases admitted to Women's Health Hospital, Assiut University p. 92
Shaimaa S Abdel-Raheem, Dalia G Mahran, Ghada S. T. Al-Attar, Mohammad H Qayed, Zein E. A. Zareh, Essam El-Din R. A. Othman
Introduction Maternal near-miss (MNM) is one of the related concepts to maternal mortality. MNM is a special category of survivors, whose stories provide unique insights and valuable information on maternal mortality. Measuring MNM beside maternal mortality and identifying its causes is essential, and should be calculated regularly for the purpose of planning, monitoring, and evaluation of provided maternal healthcare. Objectives The objectives of the study were to determine the magnitude and to identify the patterns of MNM among cases admitted to Women's Health Hospital, Assiut University, Egypt. Methodology This paper is a part of a larger case–control prospective study; however, for this analysis, we are presenting only the findings of the MNM cases. (The full profile of the cases and controls was presented in another paper.) The study was conducted at Women's Health Hospital, Assiut University and included 342 MNM cases by total coverage of all eligible cases who met the criteria of MNM identification published in WHO bulletin (2011) throughout the 12 months' period of the study. A checklist was used to collect data from the hospital records of eligible respondents. Results During the 12 months' period of the study, there were 17 503 deliveries and 16 972 live births. The maternal mortality ratio was 276 per 100 000 live births and the MNM incidence ratio was 20 per 1000 live births. This means that there was one maternal death for every seven cases of MNM. The mean age of MNM cases was 28.4 ± 8.5, whereas the mean gestational age of MNM was 35.66 ± 8.6 weeks. The main direct obstetric causes of MNM were hypertensive disorders of pregnancy (49.8%), obstetric hemorrhage (38.3%) and dystocia (32.5%). On the other hand, cardiovascular disorder was the most prevalent nonobstetric cause among MNM cases (48.8%). The peak frequency of the cases occurred during the summer season. Conclusions and recommendations MNM and maternal mortality are alarmingly high. Hypertensive disorders of pregnancy and obstetric hemorrhage were the two main direct obstetric causes of near misses that require strict and quick management protocols.
[ABSTRACT]  [HTML Full text]  [PDF]  [Mobile Full text]  [EPub]  [Sword Plugin for Repository]Beta